Bipolar Disorder, And Depression - 1616 Words

Bipolar Disorder, Manic Depressive Disorder, and Depression When looking at the difference between Bipolar Disorder, Manic Depressive Disorder, and Depression there are not many difference. One could look at the symptoms and see that with each there is a risk of moving towards the next disorder. An individual can be diagnosed with the lowest form of depression and if not taken care of can push the individual to the extreme without ever really knowing. Knowing the symptoms of each disorder, taking time to have tests run, having the diagnose given, and starting on the right track to controlling the disorder can most likely save one’s life in the grand scheme of like. When an individual thinks of Bipolar Disorder, it is believed that it†¦show more content†¦Instead of full blown Mania, you have hypomania (a less severe form of Mania). There may also be periods of depression typically last longer than periods of hypomania. The lowest level is Cyclothymic Disorder (Mayo Clinic, 2011), this also known as cyclothymic and is a mild form of Bipolar Disorder. With cyclothymic, hypomania and depression and be disruptive, but the highs and lows are not as they are with other types of Bipolar Disorder. The first level being Manic Depressive Disorder and some of the symptoms. Manic Depressive Disorder Symptoms. When one thinks of Manic Depressive disorder, an individuals might visualize someone being crazy. To a point that assumption can be right but remember what is said about making assumptions. Manic episodes can be mild or severe depending on the extent of the mania for a person. A few of the symptoms are decreased need for sleep (ex. One feels rested after only 2-3 hours), this is usually caused be from the mind not being able to rest. Sleep requires the mind to relax and someone who is manic depressive tend to have their mind at full speed at all times. Sometime the individual is more talkative than usual or pressures to keep talking, but the attention is easily drawn to

Customer Care Essay Free Essays

Introduction The organization Indali lounge means welcome to our guest as a god.it is a fine dine restaurant which is situated in 50 Baker Street, Marylebone. This restaurant has been open since 2years ago (2009). We will write a custom essay sample on Customer Care Essay or any similar topic only for you Order Now Indali lounge restaurant offers an Emotions and unique approach to traditional Indian cuisine.it has also been named number one in the top healthiest restaurant in UK and also got few comment by channel 4 because this healthy Indian cuisine which is low in oil while containing no butter, ghee or cream and instead the restaurant use fresh herbs and spices for a full flavour .www.indalilounge.co.uk. Type of customer Business man Tourists Events wedding group Customer care policies Business man 1. Conference facilities (private room) 2. Wi-Fi internet access In our restaurant it has various initiatives to attract business customer. There is only one small conference hall size range which can accommodate less than 30 guest capacity.in conference hall business got all kind of contemporary appliances including Lcd projector ,mike, flat television etc. The conference hall is made sound proof to avoid distraction for the other guests who are in restaurant. Internet access are free Wi-Fi is an attraction for business man guest in between restaurant manager provide all kind of facilities like food’s drinking’s. However in case any failure to access connectivity, printing error etc. Our restaurant also gives special offer or 20% discount on food and drinks to the business man guests. (Condition) if they are more than 10 peoples. Tourist’s. 1. Family’s 2. Disable and baby sitting 3. Accessory for children’s This restaurant is good for tourist because it situated in Baker Street near central London so it’s easy to visit. This restaurant provides breakfast and lunch buffet which is cheaper than other restaurants. Somehow they provide starters, 5 variety meals include veg and non-veg with desserts just for 9 pounds. They can give 50% discount on food if customer have taste card.in case customer doesn’t like any food or bad taste food in that case company give them complementary food, desserts, tea coffee or discount to make customer happy. Another offer is web reservation for all Sunday evening dinner reservation made though the website there is special 50% discount. This is based on A LA Carte menu and on a minimum of 2 main courses. No other offer can be used in conjunction. Extra table and baby-sitting is very valuable for tour ester. Restaurant also look after every kind of guest so they provide extra sofa baby-sitting free charge and give 1 waiter to look after hole time. The policy of restaurant for disable people’s waiter serves all food on the plate. And the restaurant also provides special sitting area which is very convenient for children and disables. Free accessory’s for children less under 10 year. This restaurant provide extra things for children to have fun like colour, drawing paper, pencils and games etc.as well as they give discount to children’s they doesn’t charge on child meal. That’s why customer attracts to this restaurant. They glade to see them happy Events wedding groups 1. Wedding package 2. Size of hall 3. Discount’s (special menu) The size of hall is plus point in our restaurant.it has more than 120 people Sitting facilities with separate dance floor and dinner buffet the table and chair are .Placed like round table, rectangular arrangement, extra space for standing peoples Etc. it has also clock room in which guest are put our lounge, clothes’ and other Extra items There is special wedding package which is made by our manager and director at Restaurant the guests will get good service. Our police is that every member of Staff has some designed and decorate hall restaurant with flowers, buckets and Other extra decorate stuff with free of cost. And there is team of professionals like Respect ion’st probably 2 our 3 to look after guests. Number of waiter’s who will Work in different sections and they serve food on the table for disable people. The restaurant gives discount on the special occasion our big event’s and marriage Like free welcome drinks after that buffet should be set according to special set Menu includes everything pre-starter to dessert our after dessert the restaurant Give complementary tea and coffee to all guests on the table. This is what my An organisation does with all the customers and every kind of guest. That’s why This restaurant is good comparative in market. Internal customer care policy Staff hygiene Indali staff discount According to Indalilounge : As a part of indali food hygiene policy, all staff Entering kitchen are prohibited from carrying any electronic gadgets such as Mobile phone into the kitchen at all time. Such personal belongings can be kept in Staff lockers Smoking is not allow to any staff member during working hour Because of direct contact with food all indali staffs is expected to work in Extremely hygiene conditions and follows a set dress code. Indali staff can have 40% discount on the full bill (Monday, Tuesday) and 30% on Saturday. Friends and family of staff can get discount up to 20% on (Monday, Tuesday at indali even when not accompanied by the staff member Wednesdays And Sunday only) and there is no discount on Thursday, Friday and family and Friends of staff can get discount up to 20% on Monday and Tuesday and indali even When not accompanied by staff member. The important of establishing effective customer care Customer care in hospitality is one of the most important roles in every organisation Even customer service is one of the most challenging and neglected area of management Getting good customer has to very important and more important to keeping existing it Our customer is like an advertisement for organisation if they good satisfied by you. According to David clutter buck and Susan Kernaghan standard play a most Important role in organisation Standard are best set in collaboration with or by the People who have to work to them they also said that environment create Standard Whether for internal or external customers’ needs it’s involve member of work group And representative of the customer there are few important activity or output to ensure That the standards set against it. relevant challenging realistic Measurable. However people also prefer to organisation by the recommendation of other people like family’s and friend’s etc Sometime customer also attract by the Policies of organisation it’s really important like if the organisation and procedures are written in a way as to restrict the faith of the customer in the products or service of the company then it may affect their confidence and willingness to give business. On the other hand if the policies are profitable and unbiased in order or in equitable manner then the customer will have more confidence to those organisation. Www. is4profit.com Service standard and way you treated is also attract customer they give more attention how you pretend them. If they going to appreciate the organisation about their hospitality and you facilities that’s means they will come back again and again. As well as customer satisfaction should be number one goal in any organisation it is easy to top management to assume that improvement in customer satisfaction scored demonstrate that organisation has become more customer oriented. Personal contact also pays an important role in any organisation it’s a kind of customer oriented strategy at the time of dealing customer try to find out customer needs and their demand try to talk your customer as much you can asked them about organisation like any feedback, what is the best thing do you like or not, any suggestion. Then according to their suggestion work on it Susan ward says that there are 8 rules for good customer service and it’s really work on organisation polices. 1. Answer your phones 2. Don’t make promises unless you will keep 3.Listen to your customer 4. Deal with complaints 5. Be helpful even if there’s no immediate profit in it 6. Train your staff to be always helpful courteous and knowledgeable 7. Take the extra step 8. Throw in something extra. GUIDE SINCE 2000. Evaluate the effectiveness of their customer care policies Customer care evaluation policy sets out an ambitious recommitment to learn as we doupdating our standards and practices to find out contemporary needs. It’s only customer who can evaluate your product. Once they evaluate customer care give the chance to improve the level and achieve better result. Evaluation policy should be developed to taking idea from different organisation or the development processional who apply their best thinking to solve some problem know we can learn more systematically from our work. We can also promoting the employees or performance evaluations this can also be good effect on the organisation. There is another method of evaluating the customer care policies is by customer feedback. The customer feedback plays an important role to evaluating customer care. It can be done in various ways like comment book getting it filled by the customer is one of the ways. Second is online customer would be comment online to visited official side and comment on th at , so that organisation should also be considered as a valuable comment on the system then we can make continuous improvements. We can do this only with evidence and data to inform our decisions and with unprecedented transparency about what we have learned and where†¦Ã¢â‚¬ ¦..www.usaid.gov Bibliography Concerned with my manager Date: 27-3-2011 at indalilounge Web address Type of customer and their policy www.indalilounge.co.uk (date visited 25-3-2011) Important of establishing effective customer care www.is4profit.com date: 30-3-2011 Evaluate the effectiveness of their customer care policies www.usiad.gov date: 2-4-2011 www.caterer.com 2. Books David clutter buck and Susan kernaghan (marketing customers count) How to cite Customer Care Essay, Essay examples

Analysis of Market Gaps in Australia for Houses - myassignmenthelp

Question: Discuss about theAnalysis of Market Gaps in Australia for Tiny Houses. Answer: Unmet customers need, or a group of potential customers is what defines a market gap or opportunities. Tiny houses is an Australian company that offers small homes for the people who desire to live with simplicity and economical kind of life considering their low income. The company can derive different opportunities in Australia to strengthen and enhance the companys growth. The following is a critical analysis in Australia about house provision opportunities. The companys marketing niche should be influenced by such factors as the customer's income level, their preferences and the age group. In Australia, relevant studies as noted by Martin and Troy (2017) shows that most of the people living in the urban centres are complaining because of high rates of rent. In Sydney, for example, most of the people earn an average income. Due to this factor, most of them can only afford average rent. The study continues to shows that the problem of unaffordable rent is too deep and widely spread in Melbourne and Brisbane. The study also claims that there are few housing units in those capitals (Martin and Troy, 2017). The tiny house company can use these gaps to their advantage and build more homes in these towns and charge affordable rates. The company should also consider building spacious houses which even a single parent living with their children can occupy and still afford to pay the rent. Tamara, a single parent from Sydney, narrates her struggl e in paying rent considering her low income of 50000$ where she spends almost half of it in paying rent. This is despite the fact that her house is the cheapest in the area where she resides (Taylor, 2017). This research connotes various opportunities for any company that can build many spacious and affordable. To this extent, therefore, Tiny house company have been favoured by the underlying factors. There is another group of people who have been affected by housing issues. This group comprises of university and college students. The houses that have been built near universities and colleges are not only a few, but they are also expensive. Because the students are not earning, it has become a challenge to them to access affordable housing. The students who are in their thousands are yearning for some cheap and affordable houses. Tiny housing company should utilize this opportunity by building additional but affordable houses near the institutions of higher learning to bridge the existing gap (Butler, 2017). This could also attract both the teaching and non-teaching staff to rent houses near their place of work. In addition to the already discussed opportunities, there is another factor of crowding. It is evident that in Australia, the areas perceived to have affordable houses are overcrowded. This has a direct correlation with health complication due to the unhealthy conditions associated with overcrowding. If more affordable houses are built, most people are willing to relocate from the crowded areas to avert the health complications and ease congestion (Anderson el., 2017. This gives the tiny housing company an opportunity to invest and utilize the market gap in Australia. The company should consider providing residing environments which are not overcrowded, in good living conditions and provide affordable housing units. The opportunities in the Australian housing market is mostly brought about by the high rates of rent as compared to low income earned by the people. There are also few houses that are available near the workplaces. Additionally, the difficulties brought about by inaccessibility of school from home and also the poor condition of the cheap apartments. The company can exploit all these opportunities to expand their market and grow their business. References Andersen, M. J., Williamson, A. B., Fernando, P., Wright, D., Redman, S. (2018). Housing conditions of urban households with Aboriginal children in NSW Australia: tenure type matters.BMC public health,18 (1), 7 Available at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5540447/ (Accessed April 22, 2018) Butler, J., (May 17, 2017). Even average Austrians are being priced out of Austrians cities. HuffPost. Available at: https://www.huffingtonpost.com.au/2017/05/16/even-average-families-are-being-priced-out-of-australias-cities_a_22094450/ (Accessed April 22, 2018) Martin, C., Troy, L., (May 17, 2017). Two pictures of rental housing stress and vulnerability zero in our area of need. The Conversation. Available at: https://theconversation.com/two-pictures-of-rental-housing-stress-and-vulnerability-zero-in-on-areas-of-need-77714 (Accessed April 22, 2018) Taylor, A., (November 28, 2017). Sydney renters continue to feel the pain of property market. The Sydney, morning herald. Available at: https://www.smh.com.au/business/companies/sydney-renters-continue-to-feel-the-pain-of-property-market-20171128-gzue0r.html (Accessed April 22, 2018)

Unhealthy Diets Essay Example For Students

Unhealthy Diets Essay Unhealthy Diets Some foods are considered unhealthy on grounds of their being high in calories (saturated fats), lack of antioxidants and fibers. Eating very high amounts of saturated fat and cholesterol, and very low amounts of fruits, vegetables, and whole grains increases a persons risk of getting numerous diseases.Although there is much controversy on the overall effects of fat on health, nearly all experts strongly recommend limiting intake of saturated fats and trans-fatty acids (found in hard margarine, commercial baked goods, and fast foods). Other fatty acids, however, appear to offer benefits. People should try to limit even reduced-fat foods and fat substitutes in their diets. Although people might believe that eating reduced-fat or fat substitute products means consuming fewer calories, this is often not always true. Many commercial, lowered-fat products have extra calories from sugar and other carbohydrates. We will write a custom essay on Unhealthy Diets specifically for you for only $16.38 $13.9/page Order now Bibliography:

3 Errors in Using Parentheses

3 Errors in Using Parentheses 3 Errors in Using Parentheses 3 Errors in Using Parentheses By Mark Nichol Parenthetical marks can cause difficulties for writers- and, as a result, for readers. In each of the following examples, parentheses are misused; discussion and revision of each sentence follow. 1. The act brings the United Kingdom into line with the Organization for Economic Co-operation and Development’s (OECD) recommendations in dealing with bribery and corruption. One writing convention is to insert an acronym or initialism of a term in parentheses after that term is introduced, after which the abbreviation, rather than the full term, is used in subsequent references. However, it is awkward to introduce a term in this manner when it appears in possessive form, with an apostrophe followed by s. In such cases, it is best to replace the possessive form with a prepositional phrase so that the parenthesis immediately follows the term itself: â€Å"The act brings the United Kingdom into line with the recommendations of the Organization for Economic Co-operation and Development (OECD) in dealing with bribery and corruption.† 2. Moving tangible and nontangible (for example, data files and intellectual property) assets is an option. Again, a parenthesis that provides more information should immediately follow the relevant word or phrase; in this case, the parenthetical refers not to â€Å"tangible and nontangible† but to assets: â€Å"Moving tangible and nontangible assets (for example, data files and intellectual property) is an option.† 3. This work does not cover all aspects of IT integration (those are laid out in a detailed IT integration plan that will take months to complete while parallel IT environments are operated). This treatment of a parenthesis is not strictly wrong, but when the parenthesis consists of a complete sentence, it is clearer to treat it as such, with the first letter of the first word capitalized and with a period preceding the close parenthesis mark (and terminal punctuation following the preceding sentence): â€Å"This work does not cover all aspects of IT integration. (Those are laid out in a detailed IT integration plan that will take months to complete while parallel IT environments are operated.)† If, however, a complete sentence in parentheses falls within another sentence, leave the first letter of the first word lowercase and omit the period. (Exception: A question mark or exclamation point at the end of the wording in parentheses should be retained regardless of where the parenthesis falls.) Want to improve your English in five minutes a day? Get a subscription and start receiving our writing tips and exercises daily! Keep learning! Browse the Punctuation category, check our popular posts, or choose a related post below:Good At, Good In, and Good WithTen Yiddish Expressions You Should KnowOne "L" or Two?

Fail

When to Take a College Course Pass/Fail Most college courses require students to take them for a grade, but not always: In some cases, students can take a few courses as pass/fail during their time in college. Whether or not thats a good choice for you depends on a variety of factors, and there are a few things you need to know before choosing a pass/fail option over the regular grading system. What Is Pass/Fail? Its exactly what it sounds like: When you take a course pass/fail, your instructor simply decides whether your work qualifies you to pass or fail the class, rather than assign you a letter grade. As a result, it isnt factored into your GPA, and it will show up on your transcript differently. Assuming you pass, you will get the full course credits, just as if you had received a letter grade. When to Take a Course Pass/Fail There are a few situations in which you may want to take a college course pass/fail: 1. You dont need the grade.  Whether youre fulfilling graduation requirements or you just want to experiment with other areas of study, youll probably have to take a few courses outside your major. You may want to consider the pass/fail option if a letter grade in one of those courses isnt necessary for earning your degree or getting into  graduate school. 2. You want to take a risk. Pass/fail courses have no bearing on your GPA - what class might you take if you didnt have to worry about it affecting your grades? Pass/fail can be a good opportunity to expand your horizons or take a class that will really challenge you. 3. You want to reduce your stress. Maintaining good grades takes a lot of hard work, and opting for a pass/fail course can relieve some of the pressure. Keep in mind your school will have deadlines by which you have to declare youre taking the course as pass/fail, so it might not be an option for avoiding a bad grade at the last minute. Your school also likely limits how many courses you can take pass/fail, so youll want to carefully plan how to take advantage of the opportunity. Other Things to Consider Make sure youre choosing pass/fail for the right reasons, not just because you want to take it easy. Youll still need to study, do the reading, complete the homework  and pass the exams. If you slack off, the fail will show up on your transcript, not to mention the possibility youll have to make up for the credits you didnt earn. Even if you withdraw  from the class to avoid failing it, that will also show up on your transcript (unless you get out of it during a drop period). Keep in mind you may not be able to enroll in  all as a pass/fail student, and before you commit to a grading system, you may want to discuss the choice with your academic advisor or a trusted mentor.

Innovative Approaches to Corporate Management Essay

Innovative Approaches to Corporate Management - Essay Example Innovative approaches are assessed based on its impact upon its profitability. Through implementing strategic management approaches in ones company, the need for customer satisfaction will be met, not only locally but world wide. Globalization of corporations evolved and exist for many reasons. There could be a need to follow the buying trends of consumers. Another is to meet the demand of customers. In todays business field,there is a rapid emergence of global buyers and sellers. Through the help of the latest technologies, communications, wire and money transfers, receiving of payments and any other activities are made possible regardless of distance, race, and currency. devising, implementing and improving a quality management system (QMS). (PCL - Petts Consulting Ltd) This model of a quality management system approach acknowledge the facts that quality results are achieved by producing a company manual, complete with work procedures and work instructions, proper recording and keeping of quality records and tracking of inputs and outputs. The HACCP System is a systematic approach to the identification, assessment of risk and severity, and control of biological, chemical and physical hazards associated with a particular food production process or practice. (Cianci, 2000) When speaking of HACCP, it is always associated with food safety. By using and by being certified with this system, the customers of the company are always assured of the safe processing of food. Corporate Social Responsibility CSR is about how companies manage the business processes to produce an overall positive impact on society. (Baker, 2006) It is the balance between the

Research Essay Paper Example | Topics and Well Written Essays - 2500 words

Essay - Research Paper Example The onset of international business growth and ease of access to information worldwide because of the improvement of technology, state boundaries became less important because geographic and social boundaries changed and became accommodative. The new era of globalization, which became popular particularly in the 1970s brought changes, opportunities and new considerations and challenges because it created a free market in the world compared to when globalization was an unknown phenomenon. Globalization has facilitated the formation of a common market all over the world based on a liberal exchange of goods, services and money. Therefore, economic globalization refers to the increase of integration of markets across political boundaries. The oil industry captures the aspect of globalization through the growth of MNEs and a free international market for oil products (Golove, 1996:135). Globalization has affected the oil industry in a variety of ways. Oil-exporting states occupy an inconsistent position in the international system because of several aspects. First, in terms of economy, these states are highly globalized and are highly dependent on international trade, labor and finance. All these states are the source of a commodity that is significantly vital to all sections of the world making them influential states in the world. However, these states are not politically globalized because they are not likely to sign major treaties or join intergovernmental organizations. Most of them defy the global norms of human rights in search of cheap labor (Mitchell, Marcel & Mitchell, 2012:23). All over the world, economic globalization is related to political globalization because economic globalization only works through international trade, business, and finance and labor exchanges. Political globalization also ensures that economic globalization grows because it opens local

History Of Ordnance Corps Essay Example for Free

History Of Ordnance Corps Essay The Ordnance Corp is one of the oldest branches of the Army, established May 14, 1812. The U. S. Army Ordnance Department established from The Revolutionary War. General George Washington, the commander of the Continental Army, appointed Ezekiel Cheever to provide ordnance support to army in July 1775. Until 1779, all the armies had Ordnance personnel moving with them. They were civilians and Soldiers, served as conductors for maintenance ammunition wagon and arms. Each conductor led five to six armorers who repaired small arms. In 1776, Board of War and Ordnance was created. In 1777, the first Ordnance facilities were established at Carlisle, Pennsylvania, and the first arsenal and armory operations at Springfield, Massachusetts. Other arsenals and armories were also established at Harpers Ferry, Philadelphia, and Watertown. On May 14, 1812, the Ordnance Department was organized by Congress. The Ordnance Department was responsibility for arms and ammunition production, acquisition, distribution, and storage for the war. In 1832, the Ordnance Corps had new responsibilities for researching and development of field services. Good work of the Ordnance Department continully to developed and tested during the Mexican War. The Mexican War became the foundation for the massive logistics. During the American War, the Ordnance Corps was really tested and developed. The Ordnance Corps brought massive procurement of weapons and supplies, and providing field support for fast moving armies. In 1898, Spain War, the Ordnance Corps first deployed overseas and provided close-combat support. During World War I, the Ordnance Corps mobilized and developed weapons systems, organized Ordnance training facilities, and established overseas supply depots. In World War II saw dramatic expansion of the Ordnance mission of production, maintenance and training. In both Korea War and Vietnam War, the Ordnance Corps provided supplies and maintenance, and was active in the development of rockets, guided missiles and satellites. In modern U. S. Army Regimental System, the Ordnance Corps is organized under the whole branch concept. The Chief of Ordnance serves as the Regimental  Commander, and the Office Chief of Ordnance serves as Headquarters Ordnance Corps. The Ordnance Corps will be always supporting the development, production, acquisition and sustainment of weapons, ammunitions, missiles, electronics, and mobility to supporting combat power to the U. S. Army. The insignia of the Ordnance Corps is yellow color metal shell and flame. The Shell and Flame is considered the oldest branch insignia in the U. S. army. The Shell and Flame had been used by European armies before its adoption by the U. S. army. In fact, it is still used by European armies. This insignia was assigned to the Ordnance Corps in 1832. The branch colors are crimson and yellow. At first, the Ordnance Corps had a red trail, like Artillery. In 1851, Crimson was assigned to the Ordnance Corps. Then in 1902, it was changed to black and scarlet color. Then finally, in 1921, crimson and yellow were assigned to the Ordnance Corps again. The Act of May 14, 1812, recognized the Ordnance Corps. In 1936, the army Institute of Heraldry redesigned and standardized version of the Shell and Flame. Redesigned Shell and Flame remains the current version.

Lady Of Shallot by Alfred Lord Tennyson :: essays research papers

Lady Of Shallot   Ã‚  Ã‚  Ã‚  Ã‚  The Lady of Shallot is an interesting poem by Alfred Lord Tennyson. In this poem he tells a story of sadness and disappointment. This story is showing how a person crushed his life somehow. My guess would be he had his heart broken and felt trapped in depression.   Ã‚  Ã‚  Ã‚  Ã‚  In the beginning he talks about the scenery and how beautiful it is but he can’t get out to go where everything is ok in Camelot. He sees people enjoying their lives and longs to join them outside in the freedom of the world. He realizes he is trapped in the castle just like a prisoner. He feels as if nobody can see or hear him.   Ã‚  Ã‚  Ã‚  Ã‚  Later, he sees all the great things Camelot has to offer him but is reminded that if he goes a curse will fall upon him. He watches the whole world pass him by but he is scared by just the threat of the curse so he stays. He realizes just how alone he is wishing he had a lover to be with, someone to keep him company. He sees a new couple deeply in love and he watches wishing someone would come along.   Ã‚  Ã‚  Ã‚  Ã‚  In the next stanzas he meets someone and is completely stunned by her beauty and charm she is perfect in his eyes. Everything she does is right to him the way she walks and talks and presents herself, but she doesn’t even know he is alive. He decides he can’t take not being with her so he leaves the castle to go find his love he knows the curse has got him but he would rather live for just one more day in love and with hope then 100 years of sadness, regret and despair.   Ã‚  Ã‚  Ã‚  Ã‚  He goes out looking for her but can’t find her then the cures begins to take him and he starts to die before he can ever tell her how he feels but he would still prefer to die like this than in the prison of a castle. Lady Of Shallot by Alfred Lord Tennyson :: essays research papers Lady Of Shallot   Ã‚  Ã‚  Ã‚  Ã‚  The Lady of Shallot is an interesting poem by Alfred Lord Tennyson. In this poem he tells a story of sadness and disappointment. This story is showing how a person crushed his life somehow. My guess would be he had his heart broken and felt trapped in depression.   Ã‚  Ã‚  Ã‚  Ã‚  In the beginning he talks about the scenery and how beautiful it is but he can’t get out to go where everything is ok in Camelot. He sees people enjoying their lives and longs to join them outside in the freedom of the world. He realizes he is trapped in the castle just like a prisoner. He feels as if nobody can see or hear him.   Ã‚  Ã‚  Ã‚  Ã‚  Later, he sees all the great things Camelot has to offer him but is reminded that if he goes a curse will fall upon him. He watches the whole world pass him by but he is scared by just the threat of the curse so he stays. He realizes just how alone he is wishing he had a lover to be with, someone to keep him company. He sees a new couple deeply in love and he watches wishing someone would come along.   Ã‚  Ã‚  Ã‚  Ã‚  In the next stanzas he meets someone and is completely stunned by her beauty and charm she is perfect in his eyes. Everything she does is right to him the way she walks and talks and presents herself, but she doesn’t even know he is alive. He decides he can’t take not being with her so he leaves the castle to go find his love he knows the curse has got him but he would rather live for just one more day in love and with hope then 100 years of sadness, regret and despair.   Ã‚  Ã‚  Ã‚  Ã‚  He goes out looking for her but can’t find her then the cures begins to take him and he starts to die before he can ever tell her how he feels but he would still prefer to die like this than in the prison of a castle.

“Macbeth”: An Exploration of the dramatic nature of Act 2 Essay

Act 2 on its own has a lot of activity going on and a lot of information that you have to churn though to finally get not only the inner meanings of what Shakespeare has written but also the story. There are various little sub headings in which we can split up Act 2. These could be language, timing, irony, tension and dramatic value. I will be looking at dramatic value and the tension which is made due to the actions or expressions which occur in the act. Firstly an overview of what happens. Well Macbeth after being hyped up by Lady Macbeth to kill the King has crazy/guilt full illusions about doing so before the deed is done but eventually kills King Duncan. Duncan’s body is found and his two sons flee Scotland as there â€Å"are daggers in men’s smiles† that in its self is a great source of dramatic tension as we see the heirs to the thrown leave Scotland in fear of their lives and making Macbeths a lot easier. Macbeth gets crowned as King of Scotland, various people are suspicious about Duncan’s death and Macduff doesn’t attend his coronation. The first piece of true tension is apparent at the very beginning of Act 2. We are reminded of the prophecy from the 3 witches from the presence of Banquo’s son Fleance because the witches said â€Å"Thou shalt get kings, though thou shall be none† which means you shall father a king but not be one yourself. This would also mean that Macbeth’s quest of becoming and staying king would mean more deaths and his biggest potential threat seems to be Fleance. So we are reminded of the prophecy and a few lines down we see Banquo ask for his sword although he is in the castle of a close friend which shows the tension and fear. Early in to Macbeth and Banquo’s conversation Banquo mentions the 3 witches and Macbeth replies â€Å"I think not of them† which is an obvious lie to us because ewe now that he is going to murder Duncan on the bases of what the witches have said. We now see the first of many guilt inspired hallucinations in the form of the dagger â€Å"A dagger of the mind, a false creation, Proceeding from the heat-oppressed brain?† this snippet suggests that Macbeth is tired, his brain overheated or again tired and it is because of that, that he can see this dagger. In this speech we also see many unreal and â€Å"unnatural† objects and thoughts. This gives a great sense of tension and shows how this illusion makes Macbeth think of all these unnatural objects mainly to show that what is about to do is unnatural, this is because it was strongly believed that a Monarch was chosen specifically by God so if he did kill Duncan he would be defying God which isn’t such a good thing to do. The bell and knocking on the door seem to prolong the inevitable (Duncan’s murder) and also builds tension in doing so because the audience are on the edge of their seats with fascination of Macbeths soliloquy and want to see what happens next. Now the murder of Duncan itself isn’t actually onstage this technique seems to be borrowed from ancient Greek tragedy’s, this of course drives the audience wild because they can’t actually see the event taking place only the events before hand and after. This definitely increases tension and now the audience have only the power of suggestion to interpretate what has happened which of course is a lot more powerful than actually physically seeing what has happened and each human mind must go wild with thought and ideas about what has happened and it will be as gruesome as it needs to be. We now make a reference to Act 1, as Macbeth are trying to rid their hands of Duncan’s blood we look back at the battle scene in Act 1 were again Macbeth is covered in blood from the long and gruesome battle. But Lady Macbeth reasons with the guilt trodden Macbeth and tells him that â€Å"a little water will clear us of this deed†, but as we see later on guilt gets the better of Lady Macbeth as she goes mad because of the guilt full illusion of blood on her hands that can’t be washed off haunt her until she finally dies. This is rather ironic considering in Act 2 Scene 2 she states â€Å"These deeds must not be thought After these ways; so, it will make us mad† which is what happens to her later on. After the deed Lady Macbeth no longer seems to be in the lime light as often and now stricken with confidence Macbeth takes centre stage. After this murderous passage we get a light comical interlude thanks to the castles porter who unlike most people is common and speaks differently compared to the nobles, it is also visible in the text because he is speaking in pros and his language suggests that he is of lesser importance than other characters of noble birth. The porter’s joke that the door of Inverness is like hell’s gate is ironic, given the cruel and bloody events that have just taken place within the castle. When he cries, â€Å"Who’s there, i’ th’ name of Beelzebub?† we see that unnatural and evil beings and instead receiving a happy or normal welcome when they step into Macbeth’s castle, people are warned that they are putting themselves in danger in this case in the hands of the Devil. Lennox later states to Macbeth at lines 49-56 of the tremendously distressful weather that night, hear we see that Macbeth has unbalanced the pure fabric of being. By defying Gods wish he has in effect left an unnatural tear in time itself, this of course angers God and he brings down over Macbeth’s castle an unnatural storm which lasted â€Å"the livelong night†. You would expect a long reply from Macbeth but because of what he has just done he tries to tone down the thought of evil and he bluntly states â€Å"Twas a rough night.† This response seems rather odd and it seems so. Soon after Duncan’s lifeless body is discovered and is if Macbeth had planned in his head the next few days he springs in to make me king mode by taking control over the situation and the nobles. This is both prompting their decision to make him king and it will also make him look less likely a candidate to kill Duncan. As Macbeth and the nobles prance around feeling sad (obviously not Macbeth he has to lie which builds up tension and fear of getting caught is being portrayed in this fashion by him trying to avoid people believing that he is not sad, and if not why well†¦ †¦..MURDERER) they stumble upon the chamberlains which thank to Lady Macbeth are covered in blood and have daggers also covered in blood in their hands. Macbeth quickly sees an easy way out of being eventually found out, he does this by shifting the blame on the innocent and unaware chamberlains. His fellow nobles don’t seem as sure as Macbeth well of course because he’s lying they didn’t kill Duncan he did, but even so as they question his suspicions Macbeth kills again and leaves the chamberlains bloodier than before and with out life. We can see how much Macbeth’s confidence has grown and how his willingness to do anything to become king has also grown and he now doesn’t seem to mind killing anyone to do so. Of course this shocks the nobles quite a bit and many get a bit edgy. Especially Macduff who later asks Macbeth why he killed the chamberlains, only to get a response from Macbeth that he was driven by passionate anger bought about from Duncan’s death. More tension surfaces in scene 4 when The Thane of Ross speaks to the old man. They speak of the unnatural behaviour referring to both the harsh weather and the odd behaviour of the animals. This of course surrounds the coronation of Macbeth suggesting again that it was not to be and that fate and God aren’t on Macbeth’s side. In the act there are many images of unnatural behaviour these are used to make you see good from evil which in itself is a conflict which brings great tension between what is supposed to be and what is not. We then see Macduff desert Macbeths castle to return to Fife instead of being at his coronation which shows obvious rivalry and conflict of interests. It is also interesting that Macduff is responsible for Macbeth’s death and not Malcolm who is the rightful heir, Malcolm should really avenge his father but he lacks courage and that killer edge that Macduff has. Also just like Duncan’s murder Macbeth’s coronation is off stage we learn of Macbeth’s new position from Macduff, Ross and the old man, so again Shakespeare denies us of seeing the inevitable for dramatic purposes.

School Based Assessment for Learning

School Based Assessment for Learning: A report: Assuring Fairness in the assessment component of school based assessment practice in Brunei Abstract This article describes a research project study in depth carried out with 3 self-selected ‘educators’ teaching English language subject in one of the secondary school in Brunei. All are supporting standardized task provided requires flexibility to cater the students’ need individually. The paper explores the educators' conceptions of and approaches to school based assessment in relation to their teaching.It sets the discussion in the context of ‘assessment for learning’ and the literature on students’ learning in general and raising students’ achievement in particular. Drawing out a number of key themes arising from the study, I compare and contrast these with the existing literature. Finally I examine some implications of this assessment for learners’ development, and ways in which w e could support learners in fostering their learning to achieve the aims of the education system of the 21st century.The researcher is seeing at different perspective of school based assessment for learning and if assessment is made for learners it should cater with different needs of each learner. Keywords: School-Based Assessment for Learners, Brunei Common Assessment Task, pedagogy, standardized 1. Introduction: School Based Assessment School Based Assessment for Learning (SBAfL) is an assessment for learning tool which focuses on obtaining feedback on individual student’s development and performance in the four important domains highlighted in SPN21.Brunei is moving towards the changes as learner is seen as a main player in education, hence to support the changes towards assessment for learning; Brunei Common Assessment Task (BCAT) is implemented. The idea of the introducing BCAT is to make a transition or pedagogical shift between the old system to a new format SBAfL â⠂¬ËœSchool-Based Assessment for Learners’ instead ‘of learners’. In order to ease the transition, the curriculum department introduced BCAT, standardized SBAfL test. This paper therefore examines / aims to highlight significant aspect of school based assessment or learning and the lapses in existing practices. â€Å"Assessment for learning is the process of seeking and interpreting evidence for use by learners and their teachers to decide where the learners are in their learning, where they need to go and how best to get there. † Assessment Reform Group (2002) 2. 1 Objectives or purposes of SBAfL The rationale behind school-based assessment We first can start by looking at the overseas. Internationally, particularly in the West, many countries and almost all the examination boards have introduced school-based assessment.In fact, some countries, SBA contributes to 100% of the total assessment of the students. Typically, however, in countries such as Austral ia and the UK, it contributes about 50%. So, you must ask that: why have these countries and increasingly, why are other countries now following this example and introducing SBA? First of all, the reason is to improve validity. It is to ensure that we can assess the full range of outcomes relevant to our subject. And this is to ensure that we are assessing those things that we are really value.But, there is another side of it; people often think that there is a bit of a trade-off between validity and reliability. Actually, what we know is that when school-based assessment is done properly, it can also contribute to reliability too. In other words, it can be ensure that the student has a more accurate assessment of their ability. Sometimes more accurate than can be obtained from a one-shot examination. So these are some of the reasons why we move to school-based assessment. 2. Review of Related LiteratureThe main research question is ‘to what extend school based assessment help students’ learning with the implementation of Brunei Common Assessment Task (standardized tasks)? Does standardized task could be used for all students? ’ The main theme identified from the previous introduction is the rationale of school based assessment. In literature part, I intended to review the significant of school-based assessment towards students’ learning. Prior to gathering the literature, I produced a list of necessary references and resources to cite from.These resources include library books, internet, journal articles, magazines and electronic books. The first step was a big step because it was not easy to locate the literature. In researching the literature, attention was drawn to the keywords: assessment task, assessment for learning, and standardised assessment. 3. 2 Assessment tasks Assessment and schooling go hand in hand. The assessment of learners and learning is a regular practice in education and are used to determine how far learners ha ve learnt or mastered an educational task or knowledge or how well an educational process has addressed its set objectives.Anikweze (2005: 2) precisely refers to Assessment as â€Å"the process of investigating the status or standard of learners’ attainment, with reference to expected outcomes that must have been specified as objectives† when it concerns learners’ output. Njabili, Abedi, Magesse and Kalole (2005:2) add that â€Å"The fundamental role of Assessment is to provide authentic and meaningful feedback for improving student learning, instructional practice and educational options† which means that Assessment is not and so should not be seen as an end it itself but a means to a justifiable end of learning†. the student’s point of view matters because of its effect on learning. From the student’s point of view, classroom assessment information is not merely information â€Å"about† himself or herself. Rather, it forms a m ajor part of his or her learning life, becoming part of the lessons he or she is expected to learn, the relationship he or she has with the teacher and the subject matter, and relationships with peers. (Brookhart, 2003, p. 6) 3. 3 Fairness in School Based Assessment Among recent trends around the globe, is the concern on fairness in matters concerning the various issues in education.In some contexts, Fairness and Equity are used synonymously. Fairness or equal opportunity in assessment relates to two issues: â€Å"†¦ fairness in . . . comparison: in essence, have the groups †¦ being tested had the same opportunity to learn? † (Uwakwe, 2005: p4). Teachers are professional and know their students best. So they can compare their students’ work done inside or outside the classroom. Then they can authenticate whether the students themselves have done the work. In this way the authenticity of SBA is guaranteed and students’ abilities can be accurately reflec ted.Assessment and grading procedures had the effect helping some students succeed now must serve to help all students succeed. The ‘Including all learners’ statement should be used by settings/schools to look at their existing arrangements and identify good practice. â€Å"Assessment ‘as Learning’ is a Professional Obligation in a previous system. Assessment for learning (AfL) should be Celebration of Learning – students should involve not abundant process† (Rick and Doug, 2008). 3. Research Design It will generally give the overview of the research design, methodology, method, selection of research participants, and data collection.The details of data account will be presented in the later part of this report. I had outlined a research focus and constructed the main research question aligned with the qualitative methodology. I had chosen semi-structured interviews for my data collection because it suited with the nature of my study. Three pa rticipants were recruited for the research. I developed understanding of the issues which emerged in the research from the coding frame after analysing the data collected using the grounded analysis procedures. Finally, I presented the extracts based on the participants’ transcripts. . 4 Main Research Question: The Brunei Common Assessment Tasks (BCATs) currently implemented in schools are formal, standardized and moderated assessment tasks that familiarise teachers and students with Assessment for Learning (AfL) best practises. Over time, teachers are expected to develop their own SBAfL tasks to better cater to the varied abilities and needs for their students. The main research question is ‘to what extend ‘SBAfL help students’ learning with the implementation of Brunei Common Assessment Task (standardized tasks)? 4. 5 Choice of MethodologyThe methodology that I selected and employed throughout the research is qualitative. I chose qualitative because it is fundamentally different from quantitative methods. This qualitative research methodology puts my research in a good position because I want to gain meaning and understanding of teachers’ experience stored, improved and implemented for future practices. This methodology I used requires special equipment to record and transcribe to gather and analyse the data in-depth. Therefore, the qualitative perspective emphasises on the ‘depth of the understanding’ of the study. 4. 6 SamplingThe sample involved 3 teachers who taught English language in one of the secondary schools in Bandar Seri Begawan. Considering their background of teaching experience, each teacher has taught English language at different length/duration in experience of teaching. Three teachers which I recognise as teacher A, teacher B who has taught 13 years in English language and teacher C which is a new experience teacher taught English subject. 4. 7 Limitation of Study There are shortcomings, condit ions or influences that cannot be controlled that place restrictions on the methodology and conclusions that I have chosen.Even though the report finished on time, it does not mean that I did not face any problem in doing this research. Initially, I intended to interview some students to see the overview, real-classroom situation. However, with school activities these students had to involved, it was difficult to gather these students to willingly involve in this study within the constraint time. Another difficulty I faced was, no permission was given in collecting students’ mark as the academic staff seen this as case sensitive. The idea was to view the overall and breakdown results of a chosen class as a reason to see students’ progress and development when onducting assessment. 4. The Account In line with the research question to investigate the students’ learning with the implementation of Brunei Common Assessment Task (standardized task) in supporting asses sment for learning, teachers were asked their views and experiences in conducting school-based assessment. Figure 1: This figure shows Coding Frame which indicates issues arise during the grounded analysis study. Two nodes were highly discussed; teacher’s view on school based assessment and impact of standardised Brunei Common Assessment Task (BCAT) for learners.The figure indicates number of times by which it is frequently mentioned by the participants, on both teachers’ view and the implication of the standardised task. When linkage as I observed it seems that the standardised BCAT is unconstructive for low achievers and all samples were agreed the assessment practice is quite summative. To make it easier to see account frequently mentioned by each participants, I breakdown the findings into Teacher A, Teacher B, and Teacher C. See graph: From the findings, all participants mentioned that assessment for learning (AfL) are beneficial for the learners.From the graph, i t says AfL are helpful for higher achievers, and could improve group of average students in their learning: â€Å"The feedback of the assessment helps them in improving. The weak students for some help them. For others it’s still struggle. † Teacher B On the other hand, another participant describes that standardized BCAT is unconstructive for low achievers: â€Å"My comments on this may not affect the whole scenario, because my year 7K, very special class that can’t read and can’t write so I’m struggling with them. So, standardized BCAT is not for them.It is wonderful test paper, the middle set and the top set is more relevant to them. If SBAfL, teacher are given freedom to do their own SBAfL, it will be much better, for me and for my students because I know their level and I know what to prepare for them. But the rules said it must be standardized, I couldn’t do anything†¦. So, BCAT wasn’t really helpful for them, for particul ar class. â€Å" Teacher A The participant seems to relate that standardized BCAT or assessment practice is quite summative and less helpful to cater students’ need: â€Å"In the test or during the test, I have to help them, as most of them will get zero.The student and the teacher couldn’t cope the extensive syllabus. The syllabus is so huge, even by selecting few pieces in the textbook didn’t help because by the end of the day I have to go to the basic spelling, writing, reading, and the teacher had to forget about the grammar, spelling first. We need to focus on the other skills. Teacher A 5. Discussion Following, the details of data account will be analysed, interpreted and discussed. There are two significant points that emerged from these findings.I integrate the key issue into one significant aspect which deal with the obstacles that the teachers face in conducting ‘assessment for learning’ in relation to standardised BCAT for learners as t o answer the main research question 5. 1 Discussion of assessment for learning in supporting lower achiever While the literature review gave a general picture on the roles of assessment, the finding have revealed that the challenging questions of how teacher conducting common assessment task.Since SBA is a new mode of assessment, education policy provides teachers with the assessment criteria and a series of examples to illustrate the standards. They also provide training for teachers. All these measures will ensure standardization among teachers. Teachers know their own students, but may not know the standards of students in other schools, there may be discrepancies in the assessment of a student’s performance by teachers from different school. The participants argued that there are reasons why assessment and grading procedures had the effect only helping some students succeed.Assessment for learning should not be seen as a professional obligation for the students if we want to achieve the aim of the education system. A view expressed by a participant shows that ‘including all learners’ statement should be used by settings/schools to look at their existing arrangements and identify good practice. Common assessment task shows very little indications what students could actually do in real world or situation; content-based assessment task less constructive in promoting critical thinking and skills needed by the nation.Hence, project-based does necessary happen and performance-based assessment. Conclusion If we are serious about raising student achievement, we have to change what happens in the classroom to enable all learners to access relevant skills, knowledge and understanding at an appropriate level. Schools may select content from or any key stage within the curriculum and use it flexibly, together with the skills framework given. In order to provide a meaningful relevant and motivating curriculum for their learners, meet the specific n eeds of their learners and further their all-round development.Recommendation: the need of flexibility It is recommended that flexible assessment activities is needed to cater the needs of different students and allow them to be assessed effectively. I would like to take this one step further and suggest that, there is a need of flexibility in assessing students’ task by considering level of the students, settings/schools must work to provide an inclusive curriculum which offers opportunities for all learners to achieve and contribute to today’s multi-ethnic. Arrangements for monitoring and evaluating the curriculum should be set out in school policy documents.The full range of professionals, learners and parents/carers can play a part to evaluate the effectiveness of the whole curriculum in the longer term. Standardization is helpful to cater some groups of students, however with the extensive syllabus; weaker students could not cope with too many common assessment ta sks. Therefore there is a need to reduce number of common assessment task to be beneficial for access relevant skills, knowledge and understanding In order to provide a meaningful relevant and motivating curriculum for all learners. Reference: * Black, P. and William, D. (2001). Inside the black box.Raising standards through classroom assessment. http://www. pdkintl. org/kappan/kbla9810. htm * Black, P ; Wiliam, D 1998, ‘Assessment and Classroom Learning’, Education: Principles, Policy and Practice, March, vol 5, no 1, pp 7-74. * Educational Leadership February 2000 Number: What Do We Mean by Results Using Rubrics to Promote Thinking and Learning Volume 57 Pages 13-18. Retrieved February 1, 2013, from http://www. pbs. org/teacherline/courses/rdla230/docs/session_2_andrade. pdf * Glasson, T. (2009). Improving Student Achievement. A practical guide to Assessment for Learning.Curriculum Corporation, Victoria, Australia. * Hong Kong Assessment and Examination Authority (HKE AA). Introduction to School-Based Assessment. Retrieved February 3, 2013, from http://www. hkeaa. edu. hk/en/SBA/ * Hong Kong Assessment and Examination Authority (HKEAA), http://www. hkeaa. edu. hk/en/SBA/ * Jim Tognolini (2007). Standards-based assessment: a tool and means to the development of human capital and capacity building in education. Australian Journal of Education, Vol. 51, No. 2, 2007, 129-145 * Phelps, Richard P. , Standardized Testing Primer. (New York, NY: Peter Lang, 2007)

Free Essays on Afterlife

The concept of the afterlife can be seen in many different perspectives. My opinion of the afterlife depends on how someone would have lived in life. If a human’s occupation in life was a serial killer who was put to death because of your crimes that were commited and therefore would be sent to heal without your skin attached to your body in a pool of hot oil. Complete horror and suffering are the only facts that would be true to this type or person. The punishment for there horrible beings is because of the lives that were taken of perfectly nice humane beings. Those souls of the ones lives that were taken will be sent to paradise. For souls that committed acts of telemarketing during their lives are also dammed to hell. As just punishment a telephone is attached in several spots on there bodies where ear’s have been placed and for eternity they sit strapped to a chair hear telemarketing scams. Paradise or heaven is exactly that. It’s a place of perfection., and everything that is in your wildest dreams. In this place everything you want to happen always comes true. Anything and everything that you wish to happen, does. All the inhabitants of paradise float around on clouds drinking little drinks with those umbrellas in them. In order to make it to this place, the soul needs to be free of all evil doings. This means that time that money was stolen from cash register at Mc. Donald needs to be repent. Although if a human with a good soul leaves earth because of suicide. They are not sent to hell or to heaven. They are sent to a totally different place. Here those people are set out to figure why they left their lives because of suicide. These individuals are chained to a walrus and run into walls. This will take place until their life on earth was suppose to end. Then they are sent for judgment and more than likely directly to hell. On the other hand if someone with an evil soul commits suicide then a automatic ticket to he... Free Essays on Afterlife Free Essays on Afterlife The concept of the afterlife can be seen in many different perspectives. My opinion of the afterlife depends on how someone would have lived in life. If a human’s occupation in life was a serial killer who was put to death because of your crimes that were commited and therefore would be sent to heal without your skin attached to your body in a pool of hot oil. Complete horror and suffering are the only facts that would be true to this type or person. The punishment for there horrible beings is because of the lives that were taken of perfectly nice humane beings. Those souls of the ones lives that were taken will be sent to paradise. For souls that committed acts of telemarketing during their lives are also dammed to hell. As just punishment a telephone is attached in several spots on there bodies where ear’s have been placed and for eternity they sit strapped to a chair hear telemarketing scams. Paradise or heaven is exactly that. It’s a place of perfection., and everything that is in your wildest dreams. In this place everything you want to happen always comes true. Anything and everything that you wish to happen, does. All the inhabitants of paradise float around on clouds drinking little drinks with those umbrellas in them. In order to make it to this place, the soul needs to be free of all evil doings. This means that time that money was stolen from cash register at Mc. Donald needs to be repent. Although if a human with a good soul leaves earth because of suicide. They are not sent to hell or to heaven. They are sent to a totally different place. Here those people are set out to figure why they left their lives because of suicide. These individuals are chained to a walrus and run into walls. This will take place until their life on earth was suppose to end. Then they are sent for judgment and more than likely directly to hell. On the other hand if someone with an evil soul commits suicide then a automatic ticket to he...

Quotes From Shakespeares The Tempest

Quotes From Shakespeare's 'The Tempest' The Tempest is a play by William Shakespeare. It was one of Shakespeares last works, produced in 1611. In The Tempest, Prospero and his daughter have lived on an island for 12 years. They were stranded on the island when Antonio usurped Prosperos rightful place as Duke of Milan. Here are few quotes from The Tempest. None that I love more than myself. You are a councilor; if you can command these elements to silence and work the peace of the presence, we will not hand a rope more - use your authority. If you cannot, give thanks you have lived so long, and make yourself ready in your cabin for the mischance of the hours, if it so hap.- William Shakespeare, The Tempest, 1.1A pox o your throat, you bawling, blasphemous, incharitable dog!- William Shakespeare, The Tempest, 1.1Now would I give a thousand furlongs of sea for an acre of barren ground, long heath, broom, furze, anything. The wills above be done, but I would fain die a dry death-William Shakespeare, The Tempest, 1.1 Canst thou rememberA time before we came unto this cell?- William Shakespeare, The Tempest, 1.2in my false brotherawakened an evil nature, and my trust,like a good parent, did beget !of himA falsehood in its contrary as greatAs my trust was, which had, indeed, no limit,A confidence sans bound...- William Shakespeare, The Tempest, 1.2LibraryWas dukedom large enough- William Shakespeare, The Tempest, 1.2good wombs have borne bad sons.- William Shakespeare, The Tempest, 1.2Would I mightBut ever see that man!- William Shakespeare, The Tempest, 1.2Hell is emptyAnd all the devils are here- William Shakespeare, The Tempest, 1.2I prithee,Remember I have done thee worthy service,Told thee no lies, made no mistakes, servedWithout or grudge or grumblings. Thou did promiseTo bate me a full year.- William Shakespeare, The Tempest, 1.2As wicked dew as eer my mother brushedWith ravens feather from unwholesome fenDrop on you both! A southwest blow on yeAnd blister you all oer!- William Shakespeare, The Tempest, 1.2 To name the bigger light and how the less- William Shakespeare, The Tempest, 1.2violateThe honour of my child.- William Shakespeare, The Tempest, 1.2You taught me language, and my profit ontIs, I know how to curse. The red plague rid youFor learning me your language!- William Shakespeare, The Tempest, 1.2Theres nothing ill can dwell in such a temple.If the ill spirit have so fair a house,Good things will strive to dwell witht.- William Shakespeare, The Tempest, 1.2Might I but through my prison once a daybehold this maid. All corners else o th earthLet liberty make use of; space enoughHave I in such a prison.- William Shakespeare, The Tempest, 1.2doublet is as fresh as the first day I wore it?- William Shakespeare, The Tempest, 2.1My lord Sebastian,The truth you speak doth lack some gentleness,And time to speak it inyou rub the soreWhen you should bring the plaster.- William Shakespeare, The Tempest, 2.1All things in common nature should produceWithout sweat or endeavor.- William Shak espeare, The Tempest, 2.1 Tis as impossible that hes undrownedas he that sleeps here swims.- William Shakespeare, The Tempest, 2.1As this Gonzalo; I myself could makeA chough of as deep chat. O, that you boreThe mind that I do, what a sleep were thisFor your advancement! Do you understand me?- William Shakespeare, The Tempest, 2.1Were I in England now, as I once was, and had but this fish painted, not a holiday-fool there but would give a piece of silver. There would this monster make a manany strange beast there makes a man. When they will not give a doit to relieve a lame beggar, they will lay out ten to see a dead Indian.- William Shakespeare, The Tempest, 2.2Four legs and two voices; a most delicate monster!- William Shakespeare, The Tempest, 2.2These be fine things, an if they be not sprites. Thats a brave god, and bears celestial liquor. I will kneel to him.- William Shakespeare, The Tempest, 2.2dropped from heaven?- William Shakespeare, The Tempest, 2.2Ill show thee the best springs; Ill pluck thee ber ries;Ill fish for thee, and get thee wood enough.A plague upon the tyrant I serve!Ill bear him no more sticks, but follow thee,thou wondrous man.- William Shakespeare, The Tempest, 2.2 The mistress which I serve quickens whats dead,and makes my labors pleasures. O, she isTen times more gentle than her fathers crabbed,And hes composed of harshness.- William Shakespeare, The Tempest, 3.1Poor worm, thou art infected!This visitation shows it.- William Shakespeare, The Tempest, 3.1O heaven, O earth, bear witness to this sound,and crown what I profess with kind eventIf I speak true; if hollowly, invertwhat best is boded me to mischief: I,Beyond all limit of what else i th world,Do love, prize, honor you.- William Shakespeare, The Tempest, 3.1As I told thee before, I am subject to a tyrant, a sorcerer that by his cunning hath cheated me of the island.- William Shakespeare, The Tempest, 3.2Act to Trinculo Thou liest, thou jesting monkey, thou! I would my valiant master would destroy thee. I do not lie- William Shakespeare, The Tempest, 3.2What, what did I? I did nothing! Ill go farther off.- William Shakespeare, The Tempest, 3.2And that most deeply to consider isThe beauty of his daughter. He himselfCalls her nonpareil. I never saw a womanBut only Sycorax, my dam, and she;But she as far surpasseth SycoraxAs greatst does least.- William Shakespeare, The Tempest, 3.2 Sometimes a thousand twangling instrumentsWill him about mine ears; and sometime voices,That if I then had waked after long sleep,Will make me sleep again, and then in dreamingThe clouds methought would open and show richesReady to drop upon me, that when I wakedI cried to dream again.-  William Shakespeare,  The Tempest, 3.2A living drollery! Now I will believethat there are unicorns; that in ArabiaThere is one tree, the phoenix throne, one phoenixAt this hour reigning there.-  William Shakespeare,  The Tempest, 3.3are worse than devils.-  William Shakespeare,  The Tempest, 3.3You are three men of sin, whom destiny,That hath to instrument this lower worldAnd what is  int, the never-surfeited seaHath caused to belch up you, and on this islandWhere man doth not inhabit-you mongst  menBeing most unfit to live. I have made you mad-  William Shakespeare,  The Tempest, 3.3All three of them are desperate: their great guilt,like poison given to work a great time after,No w gins to bite their spirits. I do beseech youThat are of suppler joints, follow them swiftly,And hinder them from what this ecstasyMay now provoke them to.-  William Shakespeare,  The Tempest, 3.3 All thy vexationsWere but my trials of thy love, and thouHast strangely stood the test. Here, afore heavenI ratify this my rich gift. O Ferdinand,Do not smile at me that I boast of her,For thou shalt find that she will outstrip all praiseAnd make it halt behind her.-  William Shakespeare,  The Tempest, 4.1Bestow upon the eyes of this young coupleSome vanity of mine art.-  William Shakespeare,  The Tempest, 4.1a contract of true love to celebrate-  William Shakespeare,  The Tempest, 4.1How does my bounteous sister? Go with meTo bless this twain, that they may prosperous be,And  honored  in their issue.-  William Shakespeare,  The Tempest, 4.1Let me live here foreverSo rare a wondered father and wifeMakes this place a paradise.-  Ã¢â‚¬â€¹William Shakespeare,  The Tempest, 4.1This is strange. Your fathers in some passionThat works him strangely.-  William Shakespeare,  The Tempest, 4.1Never till this daySaw I him so touched with anger, so distempered.-  Wil liam Shakespeare,  The Tempest, 4.1These our actors,As I foretold you, were all spirits, andAre melted into air, into thin air,And, like the baseless fabric of vision,The cloud-capped towers, the gorgeous palaces,The solemn temples, the great globe itself,Yea, all which it inherit, shall dissolveAnd, like this insubstantial pageant faded,Leave not a rack behind. We are such stuffAs dreams are made on, and our little lifeIs rounded with sleep.-  William Shakespeare,  The Tempest, 4.1 A devil, a born devil, on whose natureNurture can never stick; on whom my pains,Humanely taken, all, all lost, quite lostAnd as with age his body uglier grows,So his mind cankers. I will plague them all,Even to roaring.-  William Shakespeare,  The Tempest, 4.1There is not only disgrace and dishonor in that,monster, but an infinite loss.-  William Shakespeare,  The Tempest, 4.1I will have none  ont. We shall lose our timeAnd all be turned to barnacles, or to apesWith foreheads villainous low.-  William Shakespeare,  The Tempest, 4.1If you now beheld them, your affectionsWould become tender.-  William Shakespeare,  The Tempest, 5.1Though with their high  wrongs  I am struck to  th quick,Yet with my nobler reason gainst my furyDo I take  part.  The rarer action isIn virtue than in vengeance.-  William Shakespeare,  The Tempest, 5.1Behold, sir King,The wronged Duke of Milan, Prospero-  William Shakespeare,  The Tempest, 5.1I fear a madness held me. Thi s must crave,An if  this  be  at all, a most strange story.Thy dukedom I  resign,  and do entreatThou pardon me my wrongs. But how should ProsperoBe living, and be here?-  William Shakespeare,  The Tempest, 5.1 A daughter?O heavens, that they were living both in Naples,The King and Queen there! That they were, I wishMy  self  were  mudded in that oozy bedWhere my son lies. When did you lose your daughter?-  William Shakespeare,  The Tempest, 5.1O wonder!How many goodly creatures are there here!How beauteous mankind is! O brave new worldThat has such people  int!-  William Shakespeare,  The Tempest, 5.1Was Milan thrust from Milan that his issueShould become kings of Naples?-  William Shakespeare,  The Tempest, 5.1O look, sir, look, here is more of us!I prophesied if a gallows were on landThis fellow could not drown.To Boatswain Now, blasphemy,That swearst grace  oerboard, not an oath on the shore?Hast thou no mouth by land? What is the news?-  William Shakespeare,  The Tempest, 5.1Sir, my liege,Do not infest your mind with beating onThe strangeness of this business. At picked leisure,Which shall be shortly single, Ill resolve you,Which to you shall seem probable, of everyThese happened accidents; till then, be cheerfulAnd think of each thing well.-  William Shakespeare,  The Tempest, 5.1 Ay, that I will; and Ill be wise hereafter,And seek for grace. What a thrice-double assWas I to take this drunkard for a god,And worship this dull fool!-  William Shakespeare,  The Tempest, 5.1

William Shakespeare's Influence on his Period Research Paper

William Shakespeare's Influence on his Period - Research Paper Example Queen Elizabeth had strong foreign policies and tactical diplomacy, and this encouraged migration of from the two power states France and Spain. About the same period of Shakespeare’s career, religious reformations were taking place, and England was in the middle of the reformations as Queen Elizabeth reign gave freedom of religion between the Catholic and the Protestants. The Queen’s ambiguity on the issue of religion gave Shakespeare a good environment for his play. Shakespeare’s religion remains uncertain but some people believe he was Catholic, as were his parents (Huxley). However, Shakespeare brought up he daughter as a Protestant. Those who say he was Catholic base their arguments on his works saying that the scripts depict Catholic beliefs. Characters in his plays show their devotion to the saints. This is true, but we should remember that a playwright’s work does not necessarily represent his or her beliefs. The society in the Elizabethan times be lieved in a universal order of things, or the Great Chain of Being. All the things in the universe had their rightful place, from the angel to the plants and elements. The human race was above the animals and the plants, but the human soul and God were higher up the hierarchy. The plays by Shakespeare incorporated these beliefs (Hylton). Characters in the play follow this hierarchy, and they are punished if they do not align with the order. The serious relationships in his play follow this order of being, and commonly carry the plot of the play. An instance is in the play A Midsummer’s Night Dream where Titania is under a love spell. She is destined to fall in love with the creatures that she sets her eyes on first, which turns out to be Nick bottom, a weaver. This relationship highlights the violation of the order of being. At the end of the play, the mistakes are rectified and the Queen and king of the Fairies reunite. The other couple also reunites and they live happily ev er after. All the serious relationship that Shakespeare creates complies with the Great Chain of Being (Ackroyd 321). Hence, Shakespeare seems to be advocating to the hierarchy in the society. The English Renaissance period had interests in Greek and Latin classics. Some of his plays have classical characters and settings, like Venus and Adonis, Rape of Lucrece, and Julius Caesar. Some of the critics have analyzed the use of classical setting as effects of school study of works from Ovid, Plutarch, and Livy (AbsoluteShakespeare). Nevertheless, Shakespeare also used the tragic hero, a persona described by Socrates in classical literature. This is notable in the plays King Lear, and Macbeth. Still in the plays Macbeth and King Lear, Shakespeare reflects the issue of independent in women. Lady Macbeth mock and manipulates his husbands, in contrast to what The Courtier had stipulated. The Courtier was a handbook for the society in the Renaissance era, and stipulated that a woman’ s duty was to please a man. In King Lear, the daughters of the king influence their husbands and are not subservient to their father. The illustration of independent women in his play might have had an influence from the reign of Queen Elizabeth I, but might as well be reflecting Shakespeare’s idea of gender equality (McEvoy 230). Female characters in plays by Shakespeare violate the regulations observed by the society and succeed in their own ways. In conclusion, Shakespeare’s works import settings from outside the Elizabethan era. An analysis of his works reflects that

Operations Management Assignment Example | Topics and Well Written Essays - 1000 words

Operations Management - Assignment Example Techniques such as network analysis and critical path will be applied on McDonald’s restaurant as it is planning to set up a new franchise in Oxford town. 3.1. Linear Programming Linear programming is a technique that is used to identify the best possible outcome for the project considering the constraints or limitations the project manager or the operation manager has. Generally, the best possible outcome would be to achieve the lowest cost or to achieve the highest revenues from the project (Barlow, 2005). There are several reasons why organizations around the world use linear programming. McDonald’s can also make use of linear programming technique as they are setting up a new franchise in Oxford town. There can be different ways by which McDonald’s can use linear programming and some of the ways include: They can use this to find the optimal solution whether they should open the franchise or not in Oxford. Linear programming can be used to identify the number of staff the restaurant should keep. McDonald’s can also use linear programming to decide which products they need to offer. Therefore, McDonald’s can make use of linear programming to find the best solution and make sure that the new franchise is profitable. 3.2. ... Delays in the critical path would result the project manager in delaying in the whole project. By using network analysis, the management of McDonald’s can identify different tasks that would be required to start the new franchise at Oxford town. On the other hand, critical path would be used by the management of McDonald’s to identify the most important activities and there should not be any kind of delays in activities that are in the critical path because it would lead to the delay in the completion of the project. 3.3. Why a large McDonald’s restaurant needs Operational Planning and control Operational planning and control are of immense importance in making sure that the objectives of the project are achieved. Planning allows management to identify what and how things would be done and controlling would enable the management to analyze the performance against what has been planned. Deviations in the execution can be identified and adjusted only if the project is controlled effectively and if proper planning of the project is present (Simha and Word, 2009). Operation planning and controlling are important for every organization regardless of its size (Chase and Aquilano, 1977). Therefore, even large firms like McDonald’s would need to have proper operational plans and control. If the company does have an operational plan, it would lead to delay in the project and there might be possibilities that the project might not be completed. With proper planning, everyone including the operation or project manager would know what is required and how it would be performed and what each person is supposed to do. Controlling similarly will be of high importance to McDonald’s as the company would be able to identify and

The Dimensions of Interprofessional Practice (Reflective commentary) Essay

The Dimensions of Interprofessional Practice (Reflective commentary) - Essay Example Therefore, we requested the services of her health care service provider so that we could have necessary details. I was assigned with the task of gathering her medical history while Mrs. Weber was being provided with preliminary treatment which included necessary dressing to cover and heal her wound. Considering the history of Mrs. Weber, I called social worker handling Mrs. Weber’s case and also two specialists from falls clinic so that necessary input can be received regarding patient’s current life style, Osteoarthritis problem and fear for walking. For drafting a reflection of this event, Gibbs’ framework for reflection is being used (Oxford Brookes University, 2012). Although there are various methods available for reflective writing but Gibb’s framework provides a highly synchronized and systematic approach to elaborate, discuss and evaluate the event in the light of practical experiences and theoretical approaches. 2. Feelings Being a nurse, it beca me my responsibility to ensure that all the members of this inter-professional team that included a social worker who was Mrs. Weber’s caretaker, two specialists from falls clinic and my attending, remain in complete contact. ... 2010). This idea was a reflection of directives provided by NSF Standards 2001 by DoH. According to these standards, necessary interventions should be introduced to ensure that elderly patients are enabled of living their life in a healthy manner. Furthermore, it was important that impacts of illness and disability must be reduced and all the other barriers to healthy life must be mitigated. According to Mrs. Weber’s caretaker, she didn’t use any external mobilization support. She was taking medication from local community doctor as well as some unregistered homeopathic doctor. This intake of medicine from multiple sources made me suspicious about her medicine intake and her present health condition. 3. Evaluation Careful analysis of patient’s case revealed that Mrs. Weber had a hypotension problem that was accelerated by polypharmacy as she was taking medicines from NHS representatives and a local homeopathic doctor simultaneously (Hovard and Avery, 2004). Exces sive intake of diuretic resulted in high blood pressure leading to repetitive falls (Lewiecki and Watts, 2009). Furthermore, due to these falls, she had developed a fear of mobilization. An interesting input was given by the psychiatrist of falls clinic. According to him, diuretics increase the extent of urination. Since Mrs. Weber finds it difficult to move independently and does not use any sanitary pads, she urinates in her bed or other places resulting in low self-esteem. The social worker responsible for Mrs. Weber’s care added that Mrs. Weber prefers to remain isolated from the family, community and external contact. Due to these multiple factors, Mrs. Weber has become a patient of

Growth Characteristics of Bacteriophage

Growth Characteristics of Bacteriophage CHAPTER 4 GROWTH CHARACTERISTICS OF BACTERIOPHAGE INFECTING AQUACULTURE BACTERIAL PATHOGENS 4.1  Introduction Bacteriophage are naturally occurring viruses that predated on bacteria (Clokie et al., 2011). They self-replicate exponentially and leave the commensal flora unaffected makes them useful for industrial application (Tsonos et al., 2014). However, the high number of bacteriophage in environment (Clokie et al., 2011) provide the challenges to the discovery of the most effective phage in treating bacterial pathogens (Lindberg et al., 2014). Even there were many extensive reports on bacteriophages, the clinical outcome of therapy trials are variable (Tsonos et al., 2014). This indicates that there are still many parameters which are unclear that may contributed to efficacy of the phage therapy. Previously, the most common practice to evaluate the therapeutic efficacy of phages was from in vivo studies. However, Lindberg et al. (2014) provide the alternative to evaluate the efficacy the phage treatment. The information could be assessed from the important phage traits such as adsorption, lysis time and burst size (Ackermann et al., 2004). Besides that, there are various physical and chemical factors like temperature, pH and salinity which could determine the occurrence and stability of bacteriophage (JoÅ„czyk et al., 2011). These factors reported to cause the inactivation of phage through damage of the phage structure (head, tail or envelope) or DNA structural changes (Ackermann et al., 2004). Therefore, the next section in this study is aimed to characterize the bacteriophage isolates (VALLPKK3, VHLPKM4 and VPLPKK5) based on their adsorption profile, one step growth profile and stability to various range of temperature, pH and bile salt concentration. 4.2  Materials and Methods 4.2.1  Bacteriophage Isolates The bacteriophage isolates that were used in the third chapter were further characterized in this chapter. The bacteriophage isolates were designated as VALLPKK3, VHLPKM4 and VPLPKK5. 4.2.2  Bacteriophage Adsorption Assay The bacteriophage adsorption assay was carried out following the method described by Hsieh et al. (2011) with few modification. In adsorption test, the host bacteria was first grown to OD600 1.0 or equivalent to ~108 cfu/ml and diluted to ~105 cfu/ml with TSB media. About nine ml of the host bacteria was mixed with one ml of phage lysate (~103 pfu/ml) to MOI of 0.001. Then, 100  µl of the bacteria-phage mixture was taken to determine the initial phage titer. The mixture was then incubated at 28 °C with no agitation. After 10 min, one ml of the samples was collected and centrifuged at 16,000 xg for 2 min to precipitate the absorbed phages. The same was repeated every 10 min for a period 50 min. The count of unabsorbed free phages in the supernatant was determined. Then, the free phage particles over the initial phage particles was calculated and expressed in percentage. The accuracy of the free phage count was improved by triplicate separate experiments. 4.2.3  Bacteriophage One Step Growth The one step growth assay was determined following method described Hsieh et al. (2011) with slight modification. First, host bacteria (OD600 1.0) was diluted to ~106 cfu/ml. Then, 100  µl of bacterial suspension was mixed with 100  µl of phage lysate (~103 pfu/ml) to a 1 ml of final volume with sterile TSB media (MOI 0.001). Second, the phage was allowed to adsorb into bacterial cells for 30 min at 28 °C. Then the bacterial cells were precipitated by centrifugation at 16,000 xg for 2 min. Third, the bacterial-phage pellet was suspended in 50 ml sterile TSB. Subsequently, 1 ml of the bacterial-phage suspension was precipitated by centrifugation and plated to determine the initial phage count. Then, two sets of bacterial-phage suspension concurrently collected every 12 min for a period of 84 min (for VALLPKK3 and VPLPKK5) and 132 minutes (VHLPKM4) for the determination of latent period, eclipsed period and burst size. The first set was subjected to above treatment to determine t he latent period and burst size while the second set was added with 40  µl chloroform, mixed and incubated at 28 °C for 5 min before centrifugation to determine the eclipse period. The free phage count in the supernatant was determined in triplicate. The latent period and burst size was determined according to Middleboe et al. (2010). The eclipse period was determine according to Sillankorva et al. (2008). The accuracy of the free phage count was improved by triplicate separate experiments. 4.2.4  Bacteriophage Tolerance Test The stability of the bacteriophage isolates was test in different range of temperature, pH and bile salt concentrations. The temperature test was conducted for one hour, while, the pH and bile salt concentration tests were conducted for 24 hours. a.  Temperature Tolerance Test The stability of bacteriophage in different temperature was done following method described by Phumkhachorn and Rattanachaikunsopon (2010) with slight modification. The bacteriophage solution was set to approximately 105 pfu ml-1 in sterile phage buffer. About 900  µl of sterile phage buffer was distributed into sterile empty 1.5 ml microfuge tube. The tube was incubated in the dry bath at desirable temperature (40, 50, 60, 70, 80, 90 and 100 °C) at least for 30 minutes. After 30 minutes, about 100  µl of bacteriophage solution (~104 pfu) was added into the preheated tube and mixed immediately. The tube was incubated again at desirable temperature for an hour. After incubation, the tube was placed in ice-warm bath to cool the bacteriophage solution. The titer of the survival phage was calculated by double layer method. The percentage of surviving phage was calculated by dividing the number of survival phage over initial phage count. b.  pH Tolerance Test The stability of bacteriophage in different pH was done following method described by Hsieh et al. (2011) with slight modification. The pH of phage buffer was adjusted into desirable pH (2, 3, 4, 5, 6, 7, 8 and 9) using pH meter (brand). The phage buffer was sterilized using autoclave machine at 121 °C for 15 minutes. The bacteriophage was set to approximately 107 pfu ml-1 in sterile phage buffer. The bacteriophage suspension was diluted to 105 pfu ml-1 (1/100) in phage buffer with different pH. The initial phage count was calculated and the bacteriophage solution was incubated at room temperature for 24 hours. After incubation, the bacteriophage solution was diluted using normal phage buffer and plating to calculate the survival phage by double layer method. The percentage of surviving phage was calculated by dividing the number of survival phage over initial phage count. c.  Bile Salt Tolerance Test The stability of bacteriophage in different bile salt concentration was done following method described by previous. The stock of bile salt (Brand) in phage buffer was prepared by filter sterilized to final concentration of 5 %. Then, the phage buffer was adjusted into desirable bile concentration (5000 ppm, 6000 ppm, 7000 ppm, 8000 ppm and 9000 pm). The phage buffer which used for the dilution of bile concentration was presterilized using autoclave machine at 121 °C for 15 minutes. The bacteriophage was set to approximately 107 pfu ml-1 in sterile phage buffer. The bacteriophage suspension was diluted to 105 pfu ml-1 (1/100) in phage buffer with different bile concentration. The initial phage count was calculated by serial dilution in normal phage buffer. The treated bacteriophage solution was incubated at room temperature for 24 hours. After incubation, the bacteriophage solution was diluted again using normal phage buffer and plated to calculate the survival phage by double laye r method. The percentage of surviving phage was calculated by dividing the number of survival phage over initial phage count. 4.3  Result 4.3.1  Bacteriophage Adsorption Assay In the adsorption analysis, all isolates have two adsorption phases, rapid and slow adsorptions. The rapid adsorption of VALLPKK3 was occurred within 10 minutes where almost 80% of the phage adsorb to the host (Figure 4.1). This result was similar to the VHLPKM4 (Figure 4.2). Meanwhile, the rapid adsorption of VPLPKK5 showed that around 60 % of the phage adsorbed to the host (Figure 4.3). After 10 minutes, the slow rate was occurred to all isolates. The number of unadsorbed phages was approximately below 20% within 40 minutes in all phages. The increase of phage count in VPLPKK5 was occurred after 40 minutes. The increase in free phages after 50 minutes indicates that the newly formed phages are being release from the infected cells (Figure 4.3). Figure 4.1: Adsorption of VALLPKK3 to V. alginolyticus ATCC ® 17749TM Figure 4.2: Adsorption of VHLPKM4 to V. harveyi VHJR7 Figure 4.3: Adsorption of VPLPKK5 to V. parahaemolyticus VPHG1 4.3.2Bacteriophage One Step Growth The one step growth was performed to identify different phases of the phage infection process. During the initial stage, the phage-bacteria cell was separated from the free phage during the adsorption since the adsorption result showed the availability of free phage after 30 minutes of incubation. After the infection, the phage growth parameters (latent period, eclipse period and burst size) were determine from the average of three independent curves. The analysis showed that the latency and eclipse periods of VALLPKK3 (Figure 4.4), VHLPKM4 (Figure 4.5) and VPLPKK5 (Figure 4.6) were 48 and 36 minutes, 60 and 36 minutes and, 36 and 24 minutes, respectively. The latent period of VHLPKM4 was longer compared to VALLPKK3 and VPLPKK5. Meanwhile, the eclipse period of VALLPKK3 and VHLPKM4 was similar, while, the eclipse period of VPLPKK5 were shorter than those two isolates. The VALLPKK3, VHLPKM4 and VPLPKK5 showed a burst size of ~174, ~52 and ~180 phage per infected cell, respectively, at the 28 °C. Figure 4.4: One step growth curve of VALLPKK3 infected with Vibrio alginolyticus ATCC ® 17749TM at MOI of 0.001. The number of PFU per infected cell in untreated culture () and chloroform-treated culture () are also shown. The burst size, latent period and eclipse are indicated as B, L and E, respectively. Figure 4.5: One step growth curve of VALLPKK3 infected with Vibrio harveyi VHJR7 at MOI of 0.001. The number of PFU per infected cell in untreated culture () and chloroform-treated culture () are also shown. The burst size, latent period and eclipse are indicated as B, L and E, respectively. Figure 4.6: One step growth curve of VPLPKK5 infected with V. parahaemolyticus VPHG1 at MOI of 0.001. The number of PFU per infected cell in untreated culture () and chloroform-treated culture () are also shown. The burst size, latent period and eclipse are indicated as B, L and E, respectively. 4.3.3  Bacteriophage Tolerance Test The activity of all phage isolates was stable at 40 °C and declined at 50 °C following heating for 60 minutes. The activity was disappeared entirely when heated at more than 60 °C for 1 hour (Figure 4.7). When compared among the isolates, the activity of VHLPKM4 were decline dramatically to less than 20 % when incubated at 50 °C. The activity of VALLPKK3 and VPLPKK5 were dropped to 80% and 40%, respectively. The activity of bacteriophages VALLPKK3, VHLPKM4 and VPLPKK5 can be measured after incubation at pH 4 to pH 9, but disappear completely at pH 2 and pH 3 (Figure 4.8). When compared among isolates, the VALLPKK3 was sensitive to wide range of pH. Almost all of the VALLPKK3 activity was drop to 20 to 40 % after 24 hours incubation. Meanwhile, the activity of VHLPKM4 was decline to 60 % at pH 4 and 5, relatively stable at pH 6 to pH 8 and decline again to less than 60 % at pH 9. However, the activity of VPLPKK5 relatively stable at wide range of pH (pH 4 to pH 9). Meanwhile, the activity of VALLPKK3, VHLPKM4 and VPLPKK5 can be detected after incubated at bile salt concentration from 5000 ppm to 9000 ppm (Figure 4.9). Among the isolates, VALLPKK3 was more sensitive to the bile compared to VHLPKM4 and VPLPKK5. Figure 4.7:The temperature stability of VALLPKK3, VHLPKM4 and VPLPKK5. All isolates were incubated at various range of temperature (40 °C, 50 °C, 60 °C, 70 °C, 80 °C, 90 °C and 100 °C) for 1 hour. Data are the means from three independent experiments + SD. Figure 4.8:The temperature stability of VALLPKK3, VHLPKM4 and VPLPKK5. All isolates were incubated at various range of pH (2, 3, 4, 5, 6, 7, 8 and 9) for 24 hours. Data are the means from three independent experiments + SD. Figure 4.9:The bile salt stability of VALLPKK3, VHLPKM4 and VPLPKK5. All isolates were incubated at various range of bile salt concentration (5000, 6000, 7000, 8000 and 9000 ppm) for 24 hours. Data are the means from three independent experiments + SD. 4.4  Discussion The phage adsorption of VALLPKK3 and VHLPKM4 was fast (more than 80% after 10 minutes) compared to Vibrio phage PW2 (60% after 10 minutes) (Phumkhachorn and Rattanachaikunsopon, 2010). Meanwhile, the adsorption of VPLPKK5 was comparable to PW2. This might due to both phages were belonged to same family (Sipboviridae). However, the phage adsorption was reported dependent on various condition. According to Binetti et al. (2002), the phage adsorption was shown to be affected by the presence of ion calcium, physiological state of the cell, pH and temperature. The one step growth is a method to assess the life cycle of the phage (Middleboe et al., (2010). The latent period was the time from adsorption to the release of new progeny from host cell, and the burst size was the number of new virus particles liberated from a single bacterial cell (Bao et al., 2011). When compared to other vibriophage infecting same host species, there were difference in term of the burst size of the phages Ñ„As51 and Ñ„A318 (Liu et al., 2014). The V. alginolyticus phage VALLPKK3 showed high burst size compared to those two (72 and 10 PFU/infected cell). Similar finding with VPLPKK5. This V. parahaemolyticus phage was different to other V. parahaemolyticus phage VP-2 (15 PFU per infected cell) (Silva et al., 2014) where it showed bigger burst size (180 PFU per infected cell). Meanwhile, the VHLPKM4 showed different finding. This study showed smaller burst size and longer latent period compared to previous report on V. harveyi phages цžH17-7b and Ñ„H17-8b (Okano et al., 2007). They reported that where the latent period and burst size of Ñ„H17-7b and Ñ„H17-8b were 35 minutes and 100 particles, and 40 minutes and 170 particles, respectively. Thus, the findings showed that the life cycle of each phage isolates was different from each other. However, the significant of the differences was unclear since the dissimilarity was influenced by the host, medium, temperature and its own growth rate (Carey-Smith et al., 2006). In this study, the bacteriophages VALLPKK3 and VPLPKK5 showed a short period of latent period and large burst size. The shorter latent period and large burst size showed that the bacteriophages replicated more quickly and the new virus particle release more efficiently (Bao et al., 2011). This characteristic showed good candidacy of phage therapy (Silva et al., 2014). Finally, both adsorption and one step growth of phage are important to determine the phage fitness (Wang, 2006) since the phage fitness would determine the efficacy of the phage therapy (Lindberg et al., 2014). The stability in various stress condition were useful for the application of bacteriophage to inhibit the target bacteria (Lee et al., 2014; Krasowska et al., in press). In this study, the resistance to heat, pH and bile was investigated to determine the efficacy of those phages for biocontrol of V. alginolyticus, V. harveyi and V. parahaemolyticus infections. Phage which can withstand various environmental stress may be useful for the application in aquaculture (Phumkhachorn and Rattanachaikunsopon, 2010). The temperature is a important factor that affects bacteriophage survivability (Olson et al., 2004). It plays important roles in the bacteriophage attachment, penetration and multiplication (JoÅ„czyk et al., 2011). In this study, the result showed that all phages were stable at 40 °C. However, the viability was reduced after one hour incubation at 50 °C. All phage were completely inactivated in temperature over 60 °C. The phage in this study showed that they are sensitive to high temperature. This findings was different to the findings by Phumkhachorn and Rattanachaikunsopon (2010) where the phage can withstand high temperature. However, in the natural environment, the temperature usually fluctuated at the range of 28 to 32 °C (Albert and Ransangan, 2013). Since the isolates in this study were stable at the temperature up to 40 °C, the isolates would survive when release to natural environment. Nevertheless, the period of viability of these isolates after release to natural environment was unknown. In the natural environment, the phage was also facing the other stress factor such as pH. According to Krasowska et al. (in press), the acidity and alkalinity of environment are other important factors influencing phage stability. It was also reported that low pH influences phage aggregation and reduce their adsorption on bacteria cell (Langlet et al., 2007). Therefore, it was important to access the stability of the current phage isolates in different pH. The VALLPKK3 and VHLPKM4 showed resistance to acid (pH 4) and alkaline (pH 9). This showed that the member of Myoviridae family stable at acid and alkaline condition (Krasowska et al., in press). Similar to the other isolates, VPLPKK5 was also showed resistance to acid and alkaline condition. This is similar to the finding by Lasobras et al. (60) where the member of family Siphoviridae were most resistant to adverse conditions. However, this finding was different to phage ARà ¯Ã¢â€š ¬Ã‚ , a member of Siphoviridae, which is only a ctive in a narrow pH range (Krasowska et al., in press). The result of the phage tolerance to pH indicated that they were tolerant to wide range of pH. In aquaculture, oral administration was the most practical delivery method for immunization (Yasumoto et al., 2006) due to low cost and less stress to fish (Pal et al., 2009). However, the viability of orally administered phage might be rapidly reduced the presence other digestive compounds such as bile (Joerger et al., 2003). In this study, the phage isolates were exposed to various concentration of bile concentrations and result showed that the phage were still survived after incubation. However, there were reduction on the viability of the phage isolates which might showed the adverse effect of bile. With the addition of pH and other enzymes, the phage might not persist for long time in gut environment (Ma et al., 2008). 4.5  Conclusion In summary, the VALLPKK3, VHLPKM4 and VPLPKK5 were characterized by the growth and tolerance. The life cycle of the current isolates might be different when conducted different time and with different media. Therefore, the optimization was required for optimum phage multiplication which generally required for large scale production. This optimization was also contributed to the development of phage therapy. All phages are inactivated at high temperature but showed stability at temperature 40 °C. They are also stable at wide range of pH but not low pH. But, they could tolerate normal fish bile content. However, the study need to be conducted to collect the information of the period of phage survival in fish body. This information would be beneficial for the phage administration of disease treatment.